Ghana Journal of Science, Technology and Development http://gjstd.org/index.php/GJSTD Ghana Journal of Science, Technology and Development (GJSTD) is an online double blind peer review journal which publishes scholarly articles in all disciplines of science, technology and development and will normally be published every quarter of the year en-US Ghana Journal of Science, Technology and Development 2343-6727 <p style="text-align: justify;">Submission of a manuscript implies: that the work described has not been published before (except in the form of an abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that its publication has been approved by all co-authors, if any, as well as – tacitly or explicitly – by the responsible authorities at the institution where the work was carried out. Transfer.</p> <p style="text-align: justify;">As a publisher of the journal, we reserve full copyright ownership of the journal and all submissions published in it.</p> Microbial load on cowpea (Vigna unguiculata) husks stored under different conditions for use as supplementary feed for small ruminants http://gjstd.org/index.php/GJSTD/article/view/7 <p>A ninety- two (92) day experiment was carried out in Nyankpala to determine the microbial load on cowpea husks when stored in an enclosed room (T1) and in an airy or open shed (T2). There were 3 replicates for swabs taken on both treatments at three (3) different stages; i). at harvest time in the field, ii). during storage after transporting husks home and iii). at feeding time). The swabs were taken to the laboratory and serial dilution, inoculation of diluents onto a sterile blood agar for incubation and catalase test and gram staining were conducted. The results indicated that there was heavy, very little and no fungal growth in both treatments at the three stages of swab preparations respectively. Bacterial counts (log/cfu) at the field and during storage were slightly higher in T2 than in T1 (4.08 as against 3.76 and 5.07 as against 4.65 respectively) but there was no significant difference (P&gt;0.01). At feeding however, bacterial counts in T1 was higher and significantly different (P&lt;0.01) from that of T2 (6.23 as against 5.23). There was no fungal growth on the husks after storage on both treatments. When the cowpea husks were ready to be used, bacterial counts on the husks stored in the room were higher than that of the open shed.</p> Charlotte Abbey Jakper Naadam ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 5 1 1 5 Preliminary Evaluation of Microbial Quality of Water Stored in Earthen Pots in the Tamale Metropolis, Ghana http://gjstd.org/index.php/GJSTD/article/view/9 <p><em>The study was conducted to evaluate the microbial quality of earthen pot stored water in the Tamale Metropolis. A total of fifteen (15) samples were taken from five selected communities in the Metropolis from March to May 2013. Samples collected were conveyed to Water Research Institute laboratory, Tamale for analysis. Membrane filtration method was used to determine total coliform, faecal coliform and Escherichia coli while pour plate method was used to determine total heterotrophic bacteria (THB). The study obtained bacteria count of the earthen pot stored water that exceeded World Health Organisation and Ghana Standard Board specified limits. Total coliform values ranged from 9 to 5.84 X 10</em><em>2 </em><em>cfu / 100 ml with a mean of 2.47 x 10</em><em>2 </em><em>cfu / 100 ml. Comparative analysis shows that Escherichia coli and faecal coliform from some vending sites are significantly different (p &lt; 0.05). The study revealed that earthen pot stored water from the various communities were contaminated due to unhygienic handling practices such as dipping of hands and utensils into the storage earthen pot. It is therefore recommended that water stored in earthen pots should be covered with a well fitting lid after collection and usage in order to meet the Millennium Development Goal 7</em></p> D Adade S. J Cobbina G. Quacoo A. B. Duwiejuah ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 5 1 6 10 Effect of Vitrification Solution (PVS2) on Viability and Vigour of seeds of Amaranth (Amaranthus Hybridus) http://gjstd.org/index.php/GJSTD/article/view/10 <p style="text-align: justify;">Amaranth (<em>Amaranthus hybridus</em>). This was done as a step towards long term cryopreservation of seeds of <em>Amaranth</em>. The seeds of <em>Amaranth</em>, 100 per treatment, were exposed to PVS2 between 0 and 60 min. Data were taken on in vitro germination percentage of the seeds, percentage of moisture removed from the seeds, shoot and root length of the seedlings and their vigour index calculated. The results showed that PVS2 application did not have an adverse effect on germination percentage of the seeds when the exposure time was not more than 45 min. However, the root and shoot lengths as well as the vigor of the seedling were inhibited to a certain extent by the PVS2 application. PVS2 application dehydrated the moisture of the seeds during the exposure. About 34 to 60.7 % of the moisture was removed from the seeds by the PVS2. This work may pave the way for cryopreservation of seeds of <em>Amaranth </em>and similar seeds.</p><p> </p> Raphael Adu-Gyamfi George Nyarko Daniel Agyei-Mensah ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 5 1 11 17